Easyclone-markerfree
WebJun 23, 2016 · EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9 Mathew M Jessop-Fabre, Tadas … WebMay 11, 2016 · EasyClone-MarkerFree is a set of integrative and helper vectors that allows integration of up to six genes into pre-defined chromosomal sites via CRISPR-Cas9. No …
Easyclone-markerfree
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WebUSER & EasyClone plasmids B12407 ARO3K222L + ARO4K229L + pCfB2899 This article. S8 B12408 Ec.AROB + Ec.AROD + pCfB3034 This article ... EasyClone-MarkerFree Integrative Vector B9200 X-2 integration plasmid pCfB2899 Jessop-Fabre et al., 2016 B9201 X-3 integration plasmid pCfB3034 Jessop-Fabre et al., 2016 WebThe EasyClone-MarkerFree strategy was used to genomically integrate expression cassettes into yeast. (44) Integrative cassettes were either prepared as per the EasyClone-MarkerFree strategy or amplified by …
WebMay 11, 2016 · EasyClone-MarkerFree is a set of integrative and helper vectors that allows integration of up to six genes into pre-defined … WebJensen N, Strucko T et al (2014) EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae. FEMS Yeast Res 14(2):238–248 ... Jensen MK, Keasling JD, Borodina I (2016) EasyClone-MarkerFree: a vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol ...
WebMar 26, 2015 · The publicly available EasyClone‐MarkerFree vector suite allows for facile and highly standardized genome engineering, and should be of particular interest to researchers working on yeast chassis with limited markers available. 154 PDF View 1 excerpt, cites results WebMay 1, 2024 · A CRISPR-Cas9-based tool for targeted, markerless gene integration into the Y. lipolytica genome and the capability to integrate multiple genes without the need for marker recovery and into sites with known expression levels will enable more rapid and reliable pathway engineering in the yeast. 137 PDF
WebApr 24, 2024 · The EasyClone-MarkerFree toolkit allows the Cas9-mediated integration of up to three marker-less cassettes simultaneously 23 and was recently expanded to …
WebThe publicly available EasyClone-MarkerFree vector suite allows for facile and highly standardized genome engineering, and should be of particular interest to researchers … joshua book of bibleWebJessop-Fabre MM, Jakociunas T, Stovicek V et al (2016) EasyClone-MarkerFree: a vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J 11 (8):1110–1117 how to link to another page in excelWebDec 31, 2024 · TL;DR: The EasyClone-MarkerFree vector toolkit as discussed by the authors enables the integration of linearized expression cassettes into defined genomic loci, facilitated by CRISPR/Cas9, which is recruited to the chromosomal location by specific guide RNAs expressed from a set of gRNA helper vectors. how to link to a qr codeWebSep 26, 2024 · The protocol details assembling DNA into integration expression cassettes using the EasyClone method (Jessop-Fabre et al. 2016; Holkenbrink et al. 2024 ), and uses the red phenotype from ADE2 knockout (Ugolini and Bruschi 1996) to demonstrate how to generate and assemble guide RNA vectors for CRISPR-Cas9 genome targeting … joshua bounds holland miWebMar 15, 2024 · The resulting integrations into EasyClone-MarkerFree sites X-4 (pEDJ-22) and XI-5 (pEDJ-87) defined a new strain, Sc-10. Integration at EasyClone-MarkerFree site X-4 was verified by genomic integration … joshua borchardt shawnee ksWebThe genome-editing performed in this study was achieved using the EasyClone-MarkerFree method, previously described by Jensen et al. (2014). LAC4 and LAC12 genes, from K. lactis, and the ... how to link to a windows folderWebDescription. The EasyClone-MarkerFree Vector Set is a complete kit for the introduction of genes into 11 predetermined EasyClone Chomosomal loci. These have been validated … EasyClone 2.0: expanded toolkit of integrative vectors for stable gene … how to link to a specific tweet